Figure 4: L-pampo attenuates type I IFN secretion in vivo and polyI:C-induced IRF3 signaling. | Scientific Reports

Figure 4: L-pampo attenuates type I IFN secretion in vivo and polyI:C-induced IRF3 signaling.

From: Combination of TLR1/2 and TLR3 ligands enhances CD4+ T cell longevity and antibody responses by modulating type I IFN production

Figure 4

(a) Naïve B6 mice were immunized with 100 μg of OVA alone or in combination with alum, polyI:C, Pam3, or L-pampo as adjuvants. The spleen and serum of each immunized mouse were collected at the indicated time points, and the levels of IFN-γ, TNF-α, and IFN-α were determined using a multiplex analysis. The level of cytokines detected in the spleen supernatant was divided by the weight (g) of the spleen of that mouse. n.d., not detected. The data are represented as the mean ± SEM (n = 3); *p < 0.05; **p < 0.01; ***p < 0.001. (b,c) CD11c+ conventional dendritic cells (cDCs) isolated from flt3L-expressing-B16F10-injected mice (left column) or cells from the mouse macrophage cell line Raw264.7 (right column) were treated with polyI:C, Pam3, or L-pampo for the indicated time; western blots were performed to quantify proteins in the TBK1-IRF3 signaling pathway (b) or the AP-1/NFκB signaling pathway (c). The data are representative of two independent experiments.

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