Figure 4 | Scientific Reports

Figure 4

From: miR-564 acts as a dual inhibitor of PI3K and MAPK signaling networks and inhibits proliferation and invasion in breast cancer

Figure 4

Combinatorial knockdown of AKT2, GNA12, GYS1 and SRF mimics the inhibitory effects of miR-564 on proliferation and migration in breast cancer cells.

(A) End-point viability assay in MDA-MB-231 and MCF-7 cell lines 48 hours post-transfection with siCtrl or siCocktail. (B) Real-time cell proliferation results in MDA-MB-231 and MCF-7 cell lines transfected with siCtrl or siCocktail and incubated for 48 hours. (C) Cell cycle analysis of MDA-MB-231 cells after transfection with siCtrl or siCocktail using BrdU/7-AAD staining. (D) Western blot analysis of G1/S transition proteins in MDA-MB-231 cells transiently transfected with siCtrl or siCocktail. (E) DAPI/Phalloidin staining of MDA-MB-231 cells. Images were taken and quantified 24 hours after transient transfection with siCtrl or siCocktail. Cell morphological changes were quantified as a ratio of long axis to short axis for each cell. (F) Real-time migration and invasion analysis of MDA-MB-231 cells transfected with siCtrl or siCocktail using RTCA. (G) Expression levels of target genes in combination in less-invasive and invasive cancer cell lines from NCI60 panel. (H) Expression of target genes in combination in invasive and less-invasive breast cancer cell lines panel from GSE40059 dataset. Different probes of each gene were averaged.

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