Table 4 The results of group statistics and independent samples t-test for the putative DNA markers selected by DFA.

From: Application of subtracted gDNA microarray-assisted Bulked Segregant Analysis for rapid discovery of molecular markers associated with day-neutrality in strawberry (Fragaria x ananassa)

Flowering response group

DFA-selected markers

Day-neutral

Short-day

t-test (for two bulks)

Mean b

s

Mean b

s

t value

df

p

DN1 – SD

FaP2E11 a

743.53

252.08

259.98

148.78

5.723

22

**

FaP3F4

133.90

71.34

210.06

191.96

1.288

22

ns

FaP2D6

188.96

119.83

337.52

250.68

1.852

22

ns

FaP2B4

74.95

53.71

117.72

89.99

1.414

22

ns

FaP2D12

223.23

146.47

433.96

143.61

−3.559

22

**

FaP2G4

161.45

69.35

105.77

65.22

−2.026

22

ns

DN2 – SD

FaP2E2

85.71

48.94

232.53

172.45

−2.837

12.8

*

FaP3H2

545.45

243.59

368.65

322.27

−1.516

22

ns

FaP3E5

104.54

41.97

131.37

108.95

0.796

22

ns

FaP1E10

200.32

118.04

179.75

116.79

−0.429

22

ns

FaP3E7

202.05

85.17

296.19

232.35

1.318

22

ns

FaP2E9

40.82

25.66

67.17

30.59

2.287

22

*

DN3 – SD

FaP1A1 a

0.27

0.35

713.71

499.52

−4.948

11.0

**

FaP2A2

253.29

117.27

354.78

287.26

1.133

14.6

ns

FaP3D4

133.98

82.77

137.14

70.63

0.101

22

ns

FaP3B3

227.30

136.36

300.66

237.70

−0.927

22

ns

FaP3B10

38.87

18.90

49.46

23.85

1.206

22

ns

FaP2E9

46.73

19.48

67.17

30.59

1.953

22

ns

  1. s: Standard deviation. **Significant at p < 0.01. *Significant at p < 0.05. ns Not significant.
  2. aPutative DNA markers which displayed high Fisher’s ratio values.
  3. bMean signal-to-noise ratio (SNR) of two biological replicates and six technical replicates.