Figure 2 | Scientific Reports

Figure 2

From: Skin scarification with Plasmodium falciparum peptide vaccine using synthetic TLR agonists as adjuvants elicits malaria sporozoite neutralizing immunity

Figure 2

SS with P. falciparum CS peptide in Aldara elicits sporozoite neutralizing antibodies.

(A) Antibodies were measured in vitro using TSNA and individual serum (1:5 dilution) obtained following five SS doses of CS peptide with or without Aldara. Results are shown as mean number of transgenic PfPb parasite 18S rRNA copies in HepG2 cell cultures at 48 h. post infection as measured by real-time qPCR. MAB 2A10 (25 μg/ml), specific for P. falciparum CS repeats, provided a positive control for > 90% inhibition of parasite burden (dotted line). Negative controls included PfPb sporozoites in medium, naïve serum or with MAB 3D11 specific for P. berghei CS repeats. (B) Following five SS immunizations, mice were challenged by exposure to the bites of 10–15 PfPb infected mosquitoes. Levels of parasite 18S rRNA were measured by qPCR in extracts of liver obtained 40 h. post challenge. Results shown as mean parasite 18S rRNA copy number in each group, with dotted line indicating 90% reduction of parasite rRNA copy numbers. P < 0.05 by Kruskal-Wallis test with Dunn’s multiple comparison between groups against naïve. Error bars are SD for all qPCR replcates.

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