Figure 5 | Scientific Reports

Figure 5

From: Physapubescin selectively induces apoptosis in VHL-null renal cell carcinoma cells through down-regulation of HIF-2α and inhibits tumor growth

Figure 5

Physapubescin acts synergistically with TRAIL in VHL-null 786-O cells but not VHL wild-type Caki-2 cells.

(A,B) the combined effect of physapubescin and TRAIL on 786-O and Caki-2 cell viability. Columns, mean for percentage of cell viability relative to control (n = 3); bars, SEM. (C) The combined effect of physaubescin and TRAIL on apoptosis of 786-O cells. Cells were stained by Annexin V and PI and analyzed by flow cytometry. Data represent the means from three independent experiments. SEMs are less than 5%. (D) the combined effects of physaubescin and TRAIL on caspase-3 and PARP activation after indicated treatments 24 hours were detected by Western blotting analysis. A representative blot was shown from three independent experiments.

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