Figure 7 | Scientific Reports

Figure 7

From: Network analysis reveals common host protein/s modulating pathogenesis of neurotropic viruses

Figure 7

Validation of the proposed hypothesis in JEV infection.

DCFDA staining of Neuro2A cells in a time-dependent study of JEV infection was performed by flow cytometry analysis and the results were quantified as represented by histogram plot and graph. (a) The analysis did not involve any gating with a total 10000 event count for each sample. N-acetylcysteine (NAC) was used as a negative control in the experiment as it is known for its anti-oxidant property. Immunoblot images in (b) exhibits the expression levels of DJ-1 and LC3B in mitochondria from a time-dependent study of JEV infection. HSP60 were used as loading control for the immunoblot analyses. Co-expression of JEV Nakayama (JEV virus marker) and DJ-1 was analyzed using immunocytochemistry from JEV 24 hpi infected Neuro2A cells. Representative panel images demonstrate the results of immunocytochemistry analysis. (c) Scale = 10 μm, #represent p < 0.01. MFI = Mean Fluoroscent Intensity. (n = 3).

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