Figure 3
Enhanced expressed Wnt4 was localized in both the injured proximal and distal tubules following IRI, and almost all TUNEL positive cells and majority of Ki67 positive cells co-labeled with Wnt4.
(a) Representative images of TUNEL staining (brown) in each group (magnification, 400x). (b) Quantification of TUNEL-positive cells in each group. (c) Quantification of Ki67-positive cells in each group. (d) Representative Ki67 immunofluorescence images in each group (magnification, 200x). (e) Co-staining for TUNEL and Wnt4 (magnification, 200x). (f) Co-staining for Ki67 and Wnt4 (magnification, 200x). (g) Co-staining for Wnt4 and segment-specific tubular markers, AQP1 (h) and NCC, in injured kidneys 12 hours after IRI (magnification, 200x). All scale bars, 100 μm. *P < 0.05, **P < 0.01, ***P < 0.001 versus the sham group (n = 8 in each group).
