Figure 4
Urinary Wnt4 excretion is significantly elevated early in IRI mice, in correlation with tubular injury and urinary KIM-1.
(a) Western blot analysis and quantification of urinary Wnt4 in each group. (b) Western blot analysis and quantification of urinary KIM-1 in each group. (c) ELISA analysis of urinary Wnt4 normalized to uCr in each group. (d) ELISA analysis of urinary KIM-1 normalized to uCr in each group. (e) Correlation between urinary Wnt4 levels and tubular injury. (f) Correlation between urinary Wnt4 excretion and kidney Wnt4 expression. (g) Correlation between urinary Wnt4 and KIM-1 excretion in mice. Cropped blots are shown (full-sized blots are presented in Supplementary Fig. S5). *P < 0.05, **P < 0.01, ***P < 0.001 versus the sham group (n = 8).
