Figure 4
Smad4 suppresses the growth, invasion and angiogenesis of NB cells in vitro.
(a) western blot indicating the expression of Smad4 and HPSE in IMR32 and BE(2)-C cells stably transfected with empty vector (mock) or Smad4, and those co-transfected with HPSE. (b) colorimetric assay for the HPSE activity in NB cells stably transfected with mock or Smad4, and those co-transfected with HPSE. (c,d) MTT colorimetric and soft agar assays [representation (left) and quantification (right)] showing the viability and anchorage-independent growth capability of NB cells stably transfected with mock or Smad4, and those co-transfected with HPSE. (e) representation (left) and quantification (right) of matrigel invasion assay indicating the invasion capability of NB cells transfected with mock or Smad4, and those co-transfected with HPSE. (f) representation (left) and quantification (right) of tube formation assay showing the angiogenic capability of NB cells transfected with mock or Smad4, and those co-transfected with HPSE. *P < 0.01 vs. mock.
