Figure 3 | Scientific Reports

Figure 3

From: Novel Fluorescence Arginine Analogue as a Sensor for Direct Identification and Imaging of Nitric Oxide Synthase-like Enzymes in Plants

Figure 3

Fluorescence images of tobacco leaf epidermal cells incubated with NP1 (10 μM) containing pathogen of Pst DC3000 (OD600 = 0.2) or not by syringe injection for 6 h at room temperature taken by a DeltaVision PersonalDV system (Applied Precision) using an Olympus UPLANAPO water-immersion objective lens (60 ×/1.20 numerical aperature)36.

Filters used for NP1 were exciter (470/40 nm/nm) and emitter (520/40 nm/nm). (A) Tobacco leaves incubated with Pst DC3000. (B) Tobacco leaves incubated with NP1. (C) Tobacco leaves incubated with NP1 and Pst DC3000.

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