Figure 4 | Scientific Reports

Figure 4

From: Novel Fluorescence Arginine Analogue as a Sensor for Direct Identification and Imaging of Nitric Oxide Synthase-like Enzymes in Plants

Figure 4

Fluorescence images of tobacco leaf cells incubated with NP1 s(10 μM) containing pathogen of Pst DC3000 (OD600 = 0.2) or not by syringe injection at room temperature taken by a DeltaVision PersonalDV system (Applied Precision) using an Olympus UPLANAPO water-immersion objective lens (60 ×/1.20 numerical aperature).

Filters used for GFP were exciter (470/40 nm/nm) and emitter (520/40 nm/nm). (A–C) showed tobacco leaves incubated with NP1 and Pst DC3000 for 4 h, 6 h and 8 h, respectively. (D–F) showed tobacco leaves incubated with NP1 for 4 h, 6 h and 8 h, respectively.

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