Figure 1 | Scientific Reports

Figure 1

From: Competition between members of the tribbles pseudokinase protein family shapes their interactions with mitogen activated protein kinase pathways

Figure 1

Members of the tribbles family interact with activators of all three groups of MAPKs.

(A) A schematic diagram of TRIB mediated control of MAPK activation. (B) Plasmids expressing YFP tagged full length TRIBs or MKKs were transfected into HeLa cells. 24 hrs after transfection, intracellular TRIB expression profile was examined by fluorescent microscopy. Representative images are shown. (C) MKKs (MEK1, MKK4, 6, 7) and TRIB1-3 were fused to the V1 or V2 fragments of YFP, respectively, and co-transfected to HeLa cells. Localisation of fluorescent complexes was investigated by fluorescent microscopy. YFP fluorescent images and the corresponding phase contrast image are shown in panels B,C.

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