Figure 2 | Scientific Reports

Figure 2

From: Competition between members of the tribbles pseudokinase protein family shapes their interactions with mitogen activated protein kinase pathways

Figure 2

The central kinase-like domain of tribbles and the N-terminal region of MKKs are required for the formation of the TRIB/MAPKK complex.

(A) PCA constructs, using full length or truncated versions of TRIBs or MKKs (as indicated in the Figure) were transfected to HeLa cells to map the regions of both binding partners required for the formation of TRIB/MAPKK complex. The intracellular localisation of complexes were examined by fluorescent microscopy and listed in the Figure. (B) Schematic representation of the regions (highlighted in grey) of both TRIB and MKKs, required for complex formation. (C) Full length (w.t.) or truncated versions of TRIB2 were expressed in HeLa cells and the ability of these TRIB proteins to inhibit MEKK1 driven activation of JNK- > AP-1, as measured by an AP-1 responsive luciferase reporter, was assessed. Bar graph shows Mean ± S.D. N = 4.

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