Figure 3 | Scientific Reports

Figure 3

From: Competition between members of the tribbles pseudokinase protein family shapes their interactions with mitogen activated protein kinase pathways

Figure 3

TRIB/MAPKK complex formation is induced by inflammatory activation and MAPKs compete with tribbles for MAPKK binding.

(A) The ability of PCA to detect changes in protein complexes was verified by RelA-V1 and IκBα-V2 cotransfected HeLa cells, which were stimulated by IL-1α (0.1 nM, 60 min), 24 hrs after transfection. (*p < 0.05). (B) The impact of IL-1α (0.1 nM, 60 min) stimulation on MEK1-TRIB and MKK7-TRIB complexes was examined using flow cytometry, 24 hrs. post-transfection (*p < 0.05). (C) The relative intensity of the MEK1-TRIB1 PCA complex was analysed in the presence of an increasing dose of unlabelled ERK vs. p38 expression. N ≥ 3 (■: increasing ERK dose, ▲: increasing p38 dose). (D) The relative intensity of MEK1-TRIB PCA complexes were analysed in the presence of an increasing dose of unlabelled ERK (■: TRIB1, ▲: TRIB2, ▼: TRIB3).

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