Figure 5
An ODE model to characterise the impact of tribbles mediated inhibition of MAPK activation.
(A) A reversible competitive binding model was used to analyse FACS data presented on panel A by MLAB (Civilized Software Inc). (B) The EC50 values (f, fold to ng amount of TRIB-v2 transfected) indicate the amount of the untagged TRIB protein required to interrupt half of the MKK4V1-TRIBV2 complexes. (C) HepG2 cells were transfected with the indicated siRNA, followed by an IP for TRIB3. The level of MKK7 interacting with TRIB3 was measured by Western blot. (D) The effect of varying KTRIB on the integrated response to the E1 pulse was calculated using the ODE system. TRIB concentrations were varied over ca 105 range; time courses were simulated and integrated. (E) The integrated output dose responses were fitted with a cooperative inhibition model, the results are shown here.
