Figure 3 | Scientific Reports

Figure 3

From: Generation of Functional Cardiomyocytes from the Synoviocytes of Patients with Rheumatoid Arthritis via Induced Pluripotent Stem Cells

Figure 3

Characterisation of cardiomyocytes derived from patient-specific iPSCs.

(a) Representative line-scan images and spontaneous calcium signalling of RA-iPSC-CMs and control-iPSC-CMs showed regular calcium transients. (b) The calcium-handling properties of RA-iPSC-CMs, OA-iPSC-CMs and control-iPSC-CMs were compared by examining calcium-handling parameters. (c) Contractility measurements were recorded on single, spontaneously beating cardiomyocytes derived from RA-iPSCs and control-iPSCs. (d) Expression of calcium signalling-related genes was measured in iPSC-CMs using quantitative reverse transcription-polymerase chain reaction. Data represent mean values determined in three independent experiments ± SEM. NS, not significant; *P < 0.05; **P < 0.01. RA, rheumatoid arthritis; RA-iPSCs, induced pluripotent stem cells (iPSCs) derived from RA patients; HC, healthy control; control-iPSCs, iPSCs derived from a healthy control subject; CACNA1C, voltage-dependent L type calcium channel subunit alpha 1C; ATP2A2, cardiac muscle calcium-transporting ATPase; RYR2, cardiac ryanodine receptor 2; PLN, phospholamban.

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