Figure 3

M2BP inhibits the production of MLV and VSV-G pseudotyped lentiviral vectors.

(A) A plasmid expressing M2BP was transfected into HEK293T cells with plasmids producing the VSV-G pseudotyped lentiviral vectors indicated. At 48 h posttransfection, equal volumes of the culture supernatants were used to infect HeLa-CD4-CCR5 cells. At 48 h postinfection, cells were harvested and analyzed by FACS. The relative number of GFP positive cells in the absence of M2BP was set as 100. Data presented are means ± SD of three independent experiments. (B) A plasmid expressing M2BP was transfected into HEK293T cells with an infectious clone of MLV. A plasmid expressing GFP was included to serve as a control for transfection efficiency and sample handling. At 48 h posttransfection, the producer cells and culture supernatants were harvested for Western blotting analyses. Mock: mock transfected cells. (C) An empty vector (EV), or a plasmid expressing M2BP or RIG-I was transfected into HEK293T cells. At 36 h posttransfection, cells were infected with SINV-nanoLuc. At 24 h postinfection, the virus-containing culture supernatants were collected and used to infect HeLa-CD4-CCR5 cells for 10 h, followed by luciferase activity measurement. The relative luciferase activity in the absence of M2BP was set as 100. Data presented are means ± SD of three independent experiments.