Figure 7
From: M2BP inhibits HIV-1 virion production in a vimentin filaments-dependent manner
M2BP interferes with HIV-1 Gag localization on the plasma membrane in a vimentin filaments-dependent manner.
(A) HEK293T cells were transfected with pHIV-1-iGFPΔEnv, together with an empty vector or a plasmid expressing VIMmut, with or without a plasmid expressing M2BP. At 36 h posttransfection, cells were fixed in paraformaldehyde. M2BP-myc was stained with anti-myc antibody. Fluorescence images were obtained by confocal fluorescence microscopy. (B) Quantification of images in (A) from three independent experiments. GFP-positive cells were classified as showing punctate fluorescence or continuous fluorescence at the plasma membrane, or diffuse cytoplasmic fluorescence. (C) HEK293T cells were transfected with plasmids expressing the proteins indicated. At 36 h posttransfection, postnuclear supernatants of the cell lysates were analyzed by equilibrium flotation centrifugation, followed by Western blotting analyses. EV: empty vector.
