Figure 6
Regulation of pluripotency maintaining factors, Shh pathway, and Bcl-2 by Mang-NPs.
(A) Pancreatic CSCs were treated with Mang-NPs (0–10 μM) for 48 h. The expression of Nanog, and c-Myc was measured by the Western blot analysis. β-actin was used as a loading control. (B) Pancreatic CSCs were treated with Mang-NPs (0–10 μM) for 36 h. The expression of c-Myc, Oct-4 and Nanog was measured by q-RT-PCR. Data represent mean ± SD. * and # = significantly different from control, P < 0.05. (C) Nanog shRNA enhances the inhibitory effects of Mang-NPs on colony formation. Pan CSCs/Scrambled and CSCs/Nanog shRNA were seeded and treated with Mang-NPs (0–10 μM) for 21 days. At the end of incubation period, number of colonies were counted. Data represent mean ± SD. *, #, %, and @ = significantly different from control, P < 0.05. (D) Pancreatic CSCs were treated with Mang-NPs (0–10 μM) for 48 h. The expression of Gli1, Gli2, Patched-1, and Patched-2 was measured by the Western blot analysis. β-actin was used as a loading control. (E), Pancreatic CSCs were treated with Mang-NPs (0–10 μM) for 36 h. The expression of Bcl-2 was measured by q-RT-PCR. Data represent mean ± SD. * = significantly different from control, P < 0.05.
