Figure 8
Mang-NPs inhibits pancreatic cancer growth in KPC mice by suppressing Shh-Gli pathway, and modulating the expression of stem cells markers, pluripotency maintaining factor, EMT markers and Bcl-2, XIAP and Cyclin D1.
(A) Inhibition of pancreatic cancer growth and development in KPC mice. KPC mice were treated with Mang-NPs (20 mg/kg) for about 10 weeks. At the end of experiment, mice were sacrificed and pancreas weight was recorded. Data represent mean ± SD. * and # = significantly different from untreated control group, P < 0.05. (B) Pancreatic tissues from control and Mang-NPs-treated mice were fixed and stained with H & E. Tissue sections were visualized for the presence of PanIN (PanIN-1A, PanIN-1B, PanIN-2, and PanIN-3) lesions and PDAC. nd = not detected. (C) Liver metastasis. Liver tissues from control and Mang-NPs-treated mice were visualized for the presence of nodules. (D) Expression of stem cell marker, and pluripotency maintain factors in tumor tissues. Pancreatic cancer tissues from control and Mang-NPs treated mice were subjected to the Western blot analysis, and the expression of CD24, CD133, c-Myc, Nanog and Oct4 was measured. β-actin was used as a loading control. (E) Expression of components of Shh pathway in tumor tissues. Pancreatic cancer tissues from control and Mang-NPs treated mice were subjected to the Western blot analysis, and the expression of Gli1, Gli2, Patched1, Patched2, and Smoothened was measured. (F) Expression of Bcl2, XIAP and Cyclin D1 in tumor tissues. Pancreatic cancer tissues from control and Mang-NPs treated mice were subjected to the Western blot analysis, and the expression of Bcl2, XIAP and Cyclin D1 was measured. (G) Expression of E-cadherin, N-Cadherin, Slug, Snail, and Zeb1 in tumor tissues. Pancreatic cancer tissues from control and Mang-NPs treated mice were subjected to the Western blot analysis, and the expression of E-cadherin, N-Cadherin, Slug, Snail, and Zeb1 was measured.
