Figure 3
Developmental genes are affected by HIPSTR knockdown in HEK293 and H1BP cells.
(A) Effect of HIPSTR knockdown on the expression of TFAP2A locus genes in HEK293 cells. (B) HIPSTR knockdown does not significantly affect (p-value < 0.05, fold-change >2) the abundance of TFAP2A isoforms or pre-mRNA. (C) Heat map showing that HIPSTR knockdown in HEK293 cells leads to a significant upregulation of 377 annotated genes outside of TFAP2A locus (1% FDR, fold-change >2, also see Table S1). (D) Efficiency of HIPSTR knockdown in H1BP cells. (E) Overlap between genes differentially expressed upon HIPSTR silencing in HEK293 and H1BP cells (also see Table S2). (F) Validation of a group of genes, whose expression is significantly up- and downregulated by HIPSTR knockdown in HEK293 (top panel) and H1BP cells (bottom panel), correspondingly. (G) Heat map demonstrating that HIPSTR knockdown in H1BP cells leads to significant upregulation of 572 and downregulation of 777 genes (1% FDR, fold-change >2, also see Table S3). Data shown on (A,B,D,F) are RT-qPCR read-outs of three independent experiments, error bars represent SD; N/D – not detected; the asterisks indicate statistical significance of the expression differences (fold-change >2) calculated with two-tailed t-test, equal variance (p-value < 0.05).
