Figure 4
HIPSTR is expressed by a subset of cells in early human embryos.
(A) Mapping of RNA-seq reads from ref. 7 illustrates specific expression of HIPSTR, and not TFAP2A or TFAP2A-AS1, in 8-cell and morula-stage human embryos. (B) Mapping of the 5′-ends of transcripts with strand-specific STRT-seq data from ref. 51 shows specific expression of HIPSTR in one cell (4b2) from a 4-cell human embryo, and in eight cells (8c6 through 8i6) originating from five different 8-cell human embryos; cell names are as in ref. 51. (C,D) Re-analyses of aggregate RNA-seq data for each developmental stage from ref. 7 on (C), and from ref. 50 on (D) confirms that HIPSTR induction in early embryos is independent from TFAP2A and TFAP2A-AS1 genes. (E,F) TFAP2A pre-mRNA is not detectable in human oocytes and early embryos; re-analyses of aggregate RNA-seq data from ref. 7 on (E), and from ref. 50 on (F). (G,H) HIPSTR expression is restricted to a subset of cells in early human embryos, as inferred from RNA-seq data for 8-cell- and morula-stage embryos from ref. 7 in (G), or RNA-seq data for 8-cell-stage embryos from ref. 50 in (H); plotted are overestimated FPKM values for HIPSTR expression (see text).
