Figure 7
From: Exchange factors directly activated by cAMP mediate melanocortin 4 receptor-induced gene expression
Significant role for EPACs in MC1R-induced CRE activation.
B16F10 mouse melanoma cells were transfected with CRE reporter plasmid, serum-starved for 24 h and then stimulated or not with 1 μM of α-MSH for 4 h after 30 min pretreatment with KT-5720 (5 μM), rp-Br-cAMPs (50 μM), ESI-09 (10 or 20 μM) or the carrier DMSO (0.1% or 0.2%). Data of 5 independent experiments performed in quadruplicates were compiled expressed as the mean ± S.E.M. Asterisks indicate a significant difference between ESI-09 or DMSO-treated cells using the two-sample Student’s t-test.
