Figure 3

Perturbation of filamin’s mechanosensitivity alters ring canal actin but not localization of Kelch.

Representative images of Cher-GFP, Rhodamine-Phalloidin (actin) and Kelch labelling of stage 8 chambers are shown. Images were taken from flies with hemizygous WT-GFT or MSR mutants. Note that when compared to the WT, at this stage many ring canals have disappeared in the ΔMSR mutant and some ring canals are smaller and fragmented in the open and closed MSR mutant. The arrows show ring canals that do not appear to be associated with membranes in the open MSR mutant. Scale bars 50 μm.