Figure 2
Transcriptomic profiling by RNA-Seq of wild type and aft2 cells treated with 1 mM sodium selenite.
(A) A total number of 5415 genes with valid data for both the wild-type (dark grey circle) and the aft2 (light grey circle) strains subjected to 1 mM selenite treatment were evaluated. Genes showing induction or repression in both strains are shown as intersection of the circles. The number of genes in each category is indicated. Circles are not drawn to scale. (B) Changes in mRNA levels (log2 scale) of selected genes known to respond to oxidative stress in the wild type (open bars) and aft2 (closed bars) strains after 5 h of treatment with selenite. (C) Changes in mRNA levels for genes known to specifically respond to DNA damage60. See main text for details. (D) Exponential cultures in SC medium of wild type and aft2 cells transformed with reporter plasmids in which the respective gene promoter was fused to lacZ were treated from time 0 with 1 mM selenite. β-galactosidase activity was measured in samples at the indicated times, and for each gene the values were made relative to the respective value in untreated wild type cells. Data are expressed as mean ± SD from three independent experiments.
