Figure 4
Cells lacking AFT2 hyperaccumulate selenium compared to wild type cells.
(A) Exponential cultures in SC medium of wild type (W303-1A) or aft2 (MML1086) cells transformed with the multicopy plasmid YEplac181 (vector) or its derivatives pMM1102 (PHO4) or pMM1103 (PHO81) were serially diluted and spotted on SC plates with sodium selenite. Growth was recorded after 2 days at 30 °C. (B) Wild type and aft2 cells growing exponentially in YPD medium were treated with 1 mM sodium selenite and samples taken at the indicated periods for determination of intracellular selenium accumulation by ICP-MS. Data are expressed as mean ± SEM from six independent determinations. (Turkey-Kramer test, *p < 0.05 when compared with the wild type value at the respective treatment time).
