Figure 5
Spl2 is required for Pho2-mediated rescue of selenite hypersensitivity in aft2 cells.
(A) Exponential cultures in SC medium of wild type (W303-1A), spl2 (ASC67), aft2 (MML1086) and aft2 spl2 (ASC69) cells transformed with the multicopy plasmid YEplac181 (vector) or its derivative pMM1102 (PHO4) were serially diluted and spotted on SC plates with sodium selenite. Growth was recorded after 2 days at 30 °C. (B) The indicated strains growing exponentially in SC medium were treated with 1 mM sodium selenite for 3 hours and samples were taken for determination of intracellular selenium by ICP-MS. Data are expressed as mean ± SEM from three independent determinations. (C) Northern blot analysis of expression of SPL2 in YPD medium cultures of wild type and aft2 cells after selenite addition (1 mM, final concentration) at time 0. SNR19 is employed as loading and transfer control.
