Figure 2

Western blot analysis of the proteins expression of phospho-MeCP2-S80, S421 and non-phospho-MeCP2 from paraffin sections of proliferative diabetic retinopathy membranes and idiopathic epiretinal membranes.
(A) The extracted proteins were used in immunoblotting to detect phospho-MeCP2-S80, S421 and non-phospho-MeCP2. GAPDH was used as protein loading control. Blot is representative. (B) Relative levels of phospho-MeCP2-S421 and S80 from 3 independent experiments were quantified by measuring band intensity with Image J software. Data were normalized by comparing to the control. *P< 0.01 vs. control.