Figure 2
From: Cold tolerance is unaffected by oxygen availability despite changes in anaerobic metabolism
Results of metabolic profiling at each of the three timepoints sampled: before- (top row), during- (middle row) and after (bottom row) chill coma.
2D score plots (A,D,G) showing the projection of samples onto the first discriminant plane for each of the three timepoints. Each point is a sample (four individuals pooled), colours represent different oxygen treatments (red: 0 kPa O2, green: 2.5 kPa O2, blue: 5 kPa O2, turquoise: 10 kPa O2, pink: 21 kPa O2, yellow: 40 kPa O2) and lines indicate 95% confidence intervals. The variable importance plots (B,E,H), based on VIP scores, shows the top 15 variables that contribute to component 1 at each of the timepoints (based on Pearson correlation). The coloured boxes on the right indicate the relative concentrations of each of the metabolites in each oxygen treatment, with reds indicating highest concentrations and greens the lowest concentrations. Pattern finding analysis (C,F,I) at each timepoint sampled shows the top 25 metabolites that correlated with the increase in oxygen treatments (i.e. from 0 to 40 kPa O2). Metabolites in red are positively correlated with the increase in oxygen (i.e. highest concentrations at highest oxygen), while those in blue were negatively correlated (i.e. highest concentrations at lowest oxygen). Note that these results do not necessarily denote significance. Significant differences between oxygen treatments within each timepoint can be found in Table S3. Refer to Table S2 for compound abbreviations.
