Figure 3
Effect of insulin on circadian rhythms in the mitotic division of the diabetic corneal epithelium and the recruitment of neutrophils and γδ T-cells to the diabetic corneal limbus.
(A,B) Photographs of the dividing cells in the corneal epithelium at Zeitgeber Time (ZT) 6 from control corneas and diabetic corneas. White arrows indicate the dividing cell with paired nuclei (DAPI staining; original magnification: 400x). (C) Time course of dividing epithelial cells in control, STZ, and STZ + insulin groups. (D,E) Photomicrographs of the limbus showing control corneas and diabetic corneas at ZT 15 stained with FITC-labelled anti-Ly6g (to identify neutrophils) and PE-labelled anti-CD31 (to identify vessel endothelium). (F) Time course of neutrophil recruitment to limbus from blood in control, STZ, and STZ + insulin groups. (G,H) Photomicrographs of the limbal epithelium showing control corneas and diabetic corneas at ZT 15 stained with anti–Gl3+-PE (to identify γδ T-cells) and DAPI (to identify nuclei). (I) Time course of γδ T-cell recruitment to limbal epithelium in control, STZ, and STZ + insulin groups (n = 5 corneas per group). The grey area denotes the dark phase. *P < 0.05, **P < 0.01, STZ group vs. Control group; #P < 0.05, ##P < 0.01, STZ + insulin group vs. Control group; &P < 0.05, &&P < 0.01, STZ group vs. STZ + insulin group.
