Figure 3
Effects of a dephosphorylated cofilin (dp-cofilin) peptide on spine enlargement.
(A) Images of a dendrite stimulated by repetitive uncaging of caged glutamate. Pyramidal neurons were whole-cell patch clamped and perfused without (A-a) or with (A-b) dp-cofilin peptide. Red arrowheads mark stimulated dendritic spines. (B) Time course of the changes in spine volumes from (A-a) and (A-b). (C,D) Time course of the changes in spine volumes and averaged amplitudes of spine enlargement during 1–2 min (without the dp-cofilin peptide, 113% ± 15%, 27 spines; with peptide, 17% ± 12%, 19 spines) or 15–60 min (without peptide, 46% ± 7.7%, 31 spines; with peptide, 17% ± 3.7%, 19 spines) after inducing spine enlargement. Data represent the mean ± SEM. ***p < 0.0001, *p = 0.016 (Mann–Whitney rank sum test).
