Figure 1
From: Nicotine regulates activity of lateral habenula neurons via presynaptic and postsynaptic mechanisms
Nicotine regulates LHb activity.
(A) Brain atlas diagram for approximate location of MHb and LHb (A1). Representative images of coronal slices containing the LHb with (A2) and without MHb (A3). (B–D) Nicotine first slowed and then accelerated LHb firing. Red arrow: initial firing reduction; (E) Concentration-response of nicotine-induced % increase of firing rate, recorded in cell-attached mode, in brain slices containing both LHb and MHb (●). Nicotine (1 μM and 10 μM) caused similar increases in firing rate of LHb neurons in brain slices with removed MHb (∆). ns = no significance, with MHb vs without MHb. Number of neurons is indicated. Nicotine depolarization of LHb neurons (F), even in presence of TTX (G,H). (I,J) Nicotine-induced inward currents at a holding potential of −70 mV, and in the presence of Inhibitor Cocktail. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA.
