Figure 3
From: Nicotine regulates activity of lateral habenula neurons via presynaptic and postsynaptic mechanisms
Nicotine enhances IPSCs and EPSCs in LHb neurons.
(A,B) Nicotine action on paired evoked IPSCs (eIPSCs) (A) and evoked EPSCs (eEPSCs) (B). eIPSCs (recorded at VH = +40 mV) were abolished by gabazine (10 μM), a GABAA receptor antagonist; eEPSCs (at VH = −70 mV) were abolished by DNQX (20 μM), an AMPA receptor blocker. (C) Nicotine (10 μM) enhanced the first of PSC pairs. (D) % change in amplitude (AMP) of first PSCs and paired pulse ratio (PPR = PSC2/PSC1). (E–J) Nicotine increased spontaneous sIPSC frequency and amplitude (E–G) as well as sEPSC frequency (H–J). (K,L) Simultaneous recording of sIPSC/sIPSPs and sEPSC/sEPSPs at −40 mV under voltage-(K) and current-clamp (L). (M) Different time course of nicotine action on the frequency of sIPSC/Ps and sEPSC/Ps (n = 9). (N,O) Concentration-dependence of increased sEPSC and sIPSC frequency (N) and amplitude (O). *p < 0.05, **p < 0.01, ***p < 0.001.
