Figure 1
Anti-Xoo activity on plate assays and in field trials.
(a) Comparison of the toxicity of P. taiwanensis (Pt), P. putida (Pp), P. fluorescens (Pf), and Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) was performed on a Xoo-containing 1/2 TSB agar plate (top A). Bacteria were precultured in iron-limited media for 24 h, and then tested in antagonistic assay against Xoo with OD600 of 0.5. Three culture media (iron-limited, 1/2 TSB, and LB) were tested for toxicity to Xoo (bottom a). (b) Cell numbers of P. taiwanensis were not affected by incubation with iron-limited, 1/2 TSB, or LB media. (c) Comparison of the cell free culture supernatant of factor (m/z 1044) by LC-MS in 3 different media (iron-limited, 1/2 TSB, LB) after 24 h incubation. (d,e) Field trials of the suppression of rice blight disease by P. taiwanensis. Japonica rice Tainung 67 (TN 67) and indica rice Taichung Sen 10 (TCS 10) were inoculated with total broth and cell-free supernatant of P. taiwanensis after Xoo infection. Total broth and cell-free supernatant were made in different dilutions from 10-fold to 200-fold. Field trials were conducted in two seasons. (d) The first field trial was performed in May 2015. (e) The second field trial was performed in October 2015.
