Figure 2
From: Upregulation of CYP2S1 by oxaliplatin is associated with p53 status in colorectal cancer cell lines
Involvement of p53 in CYP2S1 induction by oxaliplatin in colorectal cancer cells.
(A) p53+/+HCT116 cells, SW480 cells, HT29 cells and p53−/− HCT116 cells were treated with oxaliplatin (20 μM) for 24 h; total protein was extracted, and the protein levels of total p53 and CYP2S1 were analyzed by western blotting. The results shown are representative of three experiments. (B) Ectopic expression of p53 markedly increased SW480 cells’ CYP2S1 transcriptional activity after Oxaliplatin treatment for 24 h. Data are represented as mean ± s.d., **p < 0.01. (C, D) Effect of oxaliplatin treated on CYP2S1 mRNA expression. Isogenic p53+/+ HCT116 and p53−/− HCT116 cells were treated with oxaliplatin for 24 h; total RNA was extracted, and CYP2S1 mRNA expression was analyzed by Taqman-based real-time RT-PCR analysis. GAPDH was used as an endogenous control. (E) Cells were treated as described in panels C and D. CYP2S1 protein expression was analyzed by western blotting. GAPDH was used as an endogenous control. Representative results from three experiments are shown.
