Figure 7
Expression of Ghrelin and Ghrelin receptor genes.
Ghrelin (Ghrl) and Ghrelin receptor (Ghsr) levels were determined by RT-qPCR in F1 wild-type 129B6 and 129C3H mice (n = 4 and n = 4, respectively) and F1 Hnf1a−/− 129B6 and 129C3H mice (n = 5 and n = 6, respectively). In (A) adult jejunum and (B) P6 pancreas, an increase in Ghrl transcripts is observed in both mutant strains compared to wild-type mice but no significant differences in expression were observed between the mutant strains. (C) By contrast, in P6 pancreas, Ghsr transcripts were decreased in mutant strains compared to the wild type counterparts. This decrease was statistically significantly higher in sensitive mutant compared resistant mutant strains (*p < 0.05). NS = non-significant. Allelic expression (pyrosequencing) of the Ghsr gene is presented in F1 129C3H mice in (D) pancreas, and (E) hypothalamus. The results are represented as a percentage of expression from each allele. n = number of animals.
