Figure 3
From: Reversible off and on switching of prion infectivity via removing and reinstalling prion sialylation
Assessing secondary structure by infrared microspectroscopy.
(A) IR spectra obtained from PrPSc material purified from brains of three Syrian Hamster infected with SSLOW (blue), or PMCAb- (red), dsPMCAb- (green) or rsPMCAb-derived SSLOW (black). IR spectra were collected for products of three independent reactions for each format, PMCAb, dsPMCAb or rsPMCAb. Each spectrum represents a min/max normalized (tyrosine band at 1515 cm−1) second derivative spectrum obtained by averaging ten individual point spectra. (B) Dendrogram analysis of conformational heterogeneity of SSLOW PrPSc material purified from brains of animals infected with SSLOW or PMCAb, dsPMCAb, or rsPMCAb reactions (n = 3 independent brains or reactions). Dendrogram obtained by hierarchical cluster analysis of the mean microspectra using the information content in the amide I region (1620–1690 cm−1), using the D value as the interspectral distance measure, and Ward’s algorithm as the clustering method.
