Figure 1

Phylogenetic diversity of tongue coat microbiomes among individuals and between LC patients and healthy subjects.

(a) Rarefaction analysis of bacterial 16S rRNA gene sequences was used to evaluate if further sequencing would likely detect additional taxa, indicated by a plateau; (b) Richness index curves that evaluate the number of samples likely required to identify additional taxa indicated by a plateau; (c) Box plots depict microbiomes diversity differences according to the Shannon index, Simpson index and invsimpson index between the LCT and HT; (d) Box plots depict microbiomes diversity differences according to the Obs index, Chao 1 index and ICE index between LCT and HT. Box parameters, the “+” symbol represents median value, and the upper and lower ranges of the box represent the 75% and 25% quartiles, respectively. LCT, liver cancer patients tongue coat; HT, healthy subjects tongue coat.