Figure 1
From: Probing the luminal microenvironment of reconstituted epithelial microtissues
Non-intrusive delivery of microparticles to the luminal compartment of a reconstituted cyst.
(a) Schematic illustration and 40X image of cuboidal (15 μm × 15 μm × 15 μm) microparticles made from polyethylene glycol diacrylate (PEGDA) and loaded with FITC-BSA. (b) Schematic illustration and 40X phase contrast image of lumenized cyst reconstituted via Sacrificial Micromolding of a Caco-2 cell-aggregate in Matrigel – a reconstituted extracellular matrix (rECM). (c) Schematic illustration (left) and 40X images (right) of how Sacrificial Micromolding into Matrigel can be used to incorporate the cuboidal PEGDA microparticles into the core of Caco-2 cell-aggregates capable of undergoing morphogenesis, while also retaining the microparticle within the luminal compartment of the cyst.
