Figure 2
From: Probing the luminal microenvironment of reconstituted epithelial microtissues
Microparticle geometry affects actin belt formation and lumen incorporation.
(a) Schematic illustration and 40X phase contrast image showing how microrods (15 μm × 15 μm × 100 μm) can be incorporated within the core of Caco-2 microtissues. (b) Representative 40X phase contrast image (left) and confocal slice (right) showing how, after one week in culture, microrods alter luminal clearing and the establishment of the characteristic actin belt lining the luminal compartment of a polarized Caco-2 cyst. (c) Schematic illustration (left) and 40X images (right) of PEG microspheres (30 μm in diameter) loaded with FITC-BSA (magenta) and incorporated in lumen. (d) 40X confocal slice with widefield inset (top right) of a Caco-2 cyst showing a continuous actin staining signal (green) and a FITC-BSA loaded PEG microsphere (magenta) in the lumen.
