Figure 2

SIRT3 protects against CLP induced mitochondrial alterations and ROS production.

Wild type (WT) and genetic deletion of SIRT3 (KO) mice received CLP surgery; sham-operated animals served as negative controls (Sham). Kidney tissues were collected at 24 h after CLP surgery. (A) SIRT3 protein of kidney tissues were analyzed by western blotting. (B) Blood urea nitrogen (BUN) and (C) serum creatinine were analyzed using commercial kits. (D) Levels of ROS in the kidney were determined using DCFH-DA. (E) Representative transmission electron micrographs of the ultrastructure of mouse proximal tubular cells obtained from resin-embedded kidney sections. Scale bars: 2 μm. Quantification of mitochondria per volume (F) and mean mitochondrial volume (G) by morphometric analysis in proximal tubular cells. All data were expressed as the mean ± SEM (n = 5–10 mice/group). *p < 0.05, **p < 0.01 versus Sham in WT or KO mice. ^#p < 0.05.