Figure 5
From: Synthesis and mechanisms of action of novel harmine derivatives as potential antitumor agents
Compound 3c promoted ROS generation in HCT116 cells.
For flow cytometry analysis, HCT116 cells were treated with the indicated concentration of compound 3c for 24 hours alone or in combination with pretreatment of 20 mM LNAC or 20 mM GSH for 2 hours. For western blotting and cell viability assay, HCT116 cells were pretreated with 20 mM LNAC, 20 mM GSH or 50 μM LY294002 for 2 hours, and then treated with 8 μM compound 3c for another 24 hours. (A) Compound 3c increased the levels of ROS in HCT116 cells. (B) Pretreatment with LNAC and GSH attenuated the production of ROS resulted from compound 3c treatment. (C,D) Pretreatment with LNAC and GSH blocked the cleavage of PARP, caspase-8, caspase-9 and caspase-3 and partially restored the phosphorylated levels of AKT and ERK. (E) The cell viability of HCT116 cells was determined by CCK8 assays after treatment with compound 3c or in combination with LY294002/LNAC/GSH and the corresponding control. *P < 0.05 compared with cells treated with compound 3c. The data represents the means ± SDs. GAPDH was used as an internal control.
