Figure 2
From: STAR: a simple TAL effector assembly reaction using isothermal assembly
Overview of each step of the 4mer assembly.
(a) Lengths of 1mer and resulting 4mer fragments at each step of the STAR protocol (before and after digestion and cleanup). (b) Starting from 4 × 1mer fragments of varying sizes (423 bp, 345 bp, 123 bp), 4mer DNA fragments are assembled in a first Gibson reaction (951 bp), misassembled products cleaned away in a Plasmidsafe DNase step, correct 4mers amplified via PCR (706 bp) and blunt end fragments for further assembly generated by SchI digest and SPRI clean up (both 481 bp). (c) Sequencing strategy for verification of 4mer assembly efficiency and example sequencing trace for Sanger sequencing.
