Figure 4
From: Fungal Aflatoxins Reduce Respiratory Mucosal Ciliary Function
AFB2 acts independently of the NPY Y2 receptor.
(a) Average traces (4–6 cultures from at least 3 patients for each condition) of CBF in sinonasal ALIs exposed to PBS (vehicle; left; black trace), 1 μM NPY (left; blue trace), or NPY in the presence of BIIE-0246 (5 μM; middle magenta trace) or Gö6983 (right green trace), followed by subsequent 10 μM ATP stimulation. (b) Average CBF trances (4–6 cultures from at least 3 patients for each condition) of sinonasal ALIs stimulated with [Leu31,Pro34]-NPY (black) and NPY-(16–36) (blue). (c) Left, bar graph showing baseline CBF after 5 min under control (PBS) conditions (1.0 ± 0.01), with NPY (0.90 ± 0.01; P < 0.05 vs control), NPY + BIIE-0246 (0.97 ± 0.01; n.s. vs. control), NPY + Gö6983 (1.02 ± 0.01; n.s. vs. control), NPY-(16–36) (0.91 ± 0.03; P < 0.05 vs control), and [Leu31,Pro34]-NPY (0.99 ± 0.01; n.s. vs. control). Middle, bar graph showing peak ATP stimulated CBF with vehicle (1.61 ± 0.1), NPY (1.17 ± 0.04; P < 0.05 vs control), NPY + BIIE-0246 (1.73 ± 0.15; n.s. vs. control) and NPY + Gö6983 (1.67 ± 0.14; n.s. vs. control). Right, bar graph showing peak CBF during forskolin stimulation with NPY-(16–36) (1.35 ± 0.05) and [Leu31,Pro34]-NPY and (1.20 ± 0.04; n.s.). (d) Average CBF trace showing sequential addition of AFB2 followed by NPY. (e) Average CBF traces showing CBF changes in response to 1 and 10 μM AFB2 followed by 1 μM ATP under control conditions (left) and in the presence of 10 μM BIIE-0246 and 10 μM antagonist G (middle) or 100 μM U73122 (right). Significances determined by 1-way ANOVA with Dunnett’s post test; *P < 0.05 vs control.
