Figure 6
From: Fungal Aflatoxins Reduce Respiratory Mucosal Ciliary Function
AFB2 reduces sinonasal epithelial NO production in response to the P. aeruginosa quorum-sensing molecule and T2R38 receptor agonist C12HSL.
(a) Average traces of RNS production (DAF-FM fluorescence) during stimulation of sinonasal epithelial cells (n = 3–4 cultures from 2 genotyped PAV/PAV [functional T2R3843] patients each) with 10 μM C12HSL under control (vehicle) conditions as well as after exposure to 10 μM AFB2 ± Gö6983 followed by exposure to S-Nitroso-N-Acetyl-D,L-Penicillamine (SNAP), a non-specific NO donor. (b) Bar graph of NO production in response to C12HSL in the presence of vehicle (243 ± 35 DAF-FM fluorescence units), AFB2 (95 ± 36 units; P < 0.05 vs vehicle) or AFB2 + Gö6983 (274 ± 56 units; n.s. vs vehicle). (c) Average traces of RNS production (DAF-FM fluorescence) during exposure of sinonasal epithelial cells (n = 4–6 cultures per condition) exposed to 10 μM ionomycin and 10 μM thapsigargin under control conditions as well as after exposure to 10 μM AFB2 ± Gö6983. (d) Bar graph of NO production from C in the presence of vehicle (325 ± 50), AFB2 (148 ± 44; P < 0.05 vs vehicle) or AFB2 + Gö6983 (330 ± 25 units; n.s. vs vehicle). Significances determined by 1-way ANOVA with Dunnett’s post test; *P < 0.05 vs control.
