Figure 7
From: Fungal Aflatoxins Reduce Respiratory Mucosal Ciliary Function
AFB2 activates PKC in A549 cells in vitro.
(a) Representative image of A549 cells transfected with CKAR, showing CFP (left) and FRET (right) signals (b) Ratiometric images showing CFP/FRET ratio at baseline (left panel), after stimulation with 10 μM AFB2 (middle panel), and after subsequent 1 μM Gö6983. (c) Average traces (mean ± SEM) of CKAR FRET/CFP ratio during stimulation with forskolin and PMA (left) and AFB2. Traces are the average of 4 (forskolin/PMA) and 9 (AFB2) experiments. Bar graph to the right shows % change in CKAR FRET/CFP ratio, which was 4.3 ± 0.2% with PMA, 3.4 ± 0.1% with AFB2, and 0.2 ± 0.09% with forskolin. Significances determined by 1-way ANOVA with Bonferroni post test; *P < 0.05 vs control.
