Figure 1
From: miR-224-3p inhibits autophagy in cervical cancer cells by targeting FIP200
miR-224-3p is up regulated in HPV (+) cervical tissues and this correlates with lowered levels of autophagy.
(A) The cervical tissues were collected for miRNA microarray analysis. A portion of the heat map of analyzed miRNAs is shown. Highly up-regulated miRNAs are shown in red, while down-regulated microRNAs are shown in green. miR-224-3p is underlined. (B) Collected tissues analyzed for miR-224-3p expression using qPCR. (C) miR-224-3p expression in cervical lesions at different stages was determined by qPCR analysis. (D,E) p62 and LC3-II levels were analyzed using lysates extracted from human cervical tissues. Expression levels of each protein relative to tubulin are represented as a bar graph in (E) Data is represented as mean ± SD of n = 3 independent experiments. The boxes represent the lower and upper quartile and median; whiskers illustrate the 10th to 90th percentiles of the samples. *p < 0.05, as determined by student’s 2-tailed t-test or one-way ANOVA.
