Figure 2
From: miR-224-3p inhibits autophagy in cervical cancer cells by targeting FIP200
miR-224-3p induces autophagy in HPV(−)/(+) cervical cancer cells.
(A) Cells harvested for miR224-3p expression quantification using qPCR. (B,C) C33A, SiHa and HeLa cells were cultured under the same conditions. LC3-II, p62 (SQSTM1) and tubulin levels were determined by Western blot. The expression levels of LC3-II and p62 were normalized to tubulin. (D,E) p62 levels in the C33A, SiHa and Hela cells were analyzed via immunofluorescence. Nuclei were stained using DAPI. Representative images are shown. (F,G) LC3-II levels in the C33A, SiHa and HeLa cells were analyzed via immunofluorescence. Nuclei were stained by DAPI. Representative images are shown. The data shown are represented as mean ± SD of. n = 3 independent experiments. *p < 0.05 as determined by one-way ANOVA.
