Figure 3
From: miR-224-3p inhibits autophagy in cervical cancer cells by targeting FIP200
Ectopic expression of miR-224-3p inhibits autophagy in cervical cancer cells regardless of HPV status, but is more prominent in HPV(+) cells, especially in HPV18(+) cells.
(A,B). C33A, SiHa and HeLa cells were transfected with miR-224-3p mimicsor NCm (100 nM) for 24 h. p62 and tubulin levels were determined by Western blot. (C,D). C33A, SiHa and HeLa were treated with miR-224-3p mimicsor NCm (100 nM) for 24 h and fixed. p62 levels were analyzed by immunofluorescence. Nuclei were stained with DAPI. Representative images are shown. Data is represented as mean ± SD of n = 3 independent experiments. *p < 0.05 as determined by student’s 2-tailed t-test.
