Figure 5
From: miR-224-3p inhibits autophagy in cervical cancer cells by targeting FIP200
FIP200 is a target of miR-224-3p.
(A) Predicted binding sequences for miR224-3p in the FIP200 3′-UTR. (B) Dual-luciferase reporter vectors containing the wild-type or mutated 3′-UTR fragments of FIP200 cloned into the pmirGLO-luciferase plasmids. Luciferase activity was assayed 48 h after co-transfection with either wild-type (WT) or mutated (MUT) 3′-UTR containing plasmids and miR224m or NCm in HeLa cells. Data is represented as mean ± SD of n = 3 independent experiments. miR-224m = miR-224-3p mimetic; NCm = miRNA mimicsnegative control. *p < 0.05 as determined using student’s 2-tailed t-test.
